Just POSSIBLE presence, so far.
"First evidence of the globally threatened cave amphibian species in Montenegro: traces of Proteus environmental DNA discovered in two karst springs."
• The springs Sopot (Boka Kotorska) and Izvor Grahovo 1 (Grahovo Polje) showed weak signals on (one positive signal in three replicates) only one of the genes and were negative in the rerun; they are therefore interpreted as suspicious for the presence of Proteus (possibly on the limit of detection, although we cannot completely exclude contamination)
• Šanik spring showed a week signal on the 16S rDNA gene only, and the signal was observed again in the rerun; this sample is interpreted as plausible to contain traces of Proteus eDNA. Here contamination is less likely as the signal was observed in independent runs.
See the full report:
http://www.cepf.net/SiteCollectionDocum ... rt_SCB.pdf